Younossi, Z. M. et al. Global epidemiology of nonalcoholic fatty liver disease-Meta-analytic assessment of prevalence, incidence, and outcomes. Hepatology 64, 73–84 (2016).
Younossi, Z. et al. Global burden of NAFLD and NASH: trends, predictions, risk factors and prevention. Nat. Rev. Gastroenterol. Hepatol. 15, 11–20 (2018).
Ward, Z. J. et al. Projected U.S. state-level prevalence of adult obesity and severe obesity. N. Engl. J. Med. 381, 2440–2450 (2019).
Satapathy, S. K. & Sanyal, A. J. Epidemiology and natural history of nonalcoholic fatty liver disease. Semin. Liver Dis. 35, 221–235 (2015).
Brunt, E. M. et al. Nonalcoholic fatty liver disease. Nat. Rev. Dis. Primers 1, 15080 (2015).
Kleiner, D. E. On beyond staging and grading: liver biopsy evaluation in a posttreatment world. Hepatology 65, 1432–1434 (2017).
Rockey, D. C., Caldwell, S. H., Goodman, Z. D., Nelson, R. C. & Smith, A. D. Liver biopsy. Hepatology 49, 1017–1044 (2009).
Dietrich, C. F. et al. EFSUMB guidelines and recommendations on the clinical use of liver ultrasound elastography. Ultraschall Med. 38, e16–e47 (2017).
Masarone, M. et al. Role of oxidative stress in pathophysiology of nonalcoholic fatty liver disease. Oxid. Med. Cell. Longev. 2018, 9547613 (2018).
Koyama, Y. & Brenner, D. A. Liver inflammation and fibrosis. J. Clin. Invest. 127, 55–64 (2017).
Marcellin, P. & Kutala, B. K. Liver diseases: a major, neglected global public health problem requiring urgent actions and large-scale screening. Liver Int. 38(Suppl. 1), 2–6 (2018).
Vishwanath, K. & Ramanujam, N. in Encyclopedia of Analytical Chemistry (ed. Meyers, R.A.) 20–56 (John Wiley & Sons, 2011).
Croce, A. C., Ferrigno, A., Bottiroli, G. & Vairetti, M. Autofluorescence-based optical biopsy: an effective diagnostic tool in hepatology. Liver Int. 38, 1160–1174 (2018).
Monici, M. Cell and tissue autofluorescence research and diagnostic applications. Biotechnol. Annu. Rev. 11, 227–256 (2005).
Frangioni, J. V. In vivo near-infrared fluorescence imaging. Curr. Opin. Chem. Biol. 7, 626–634 (2003).
Ntziachristos, V., Ripoll, J. & Weissleder, R. Would near-infrared fluorescence signals propagate through large human organs for clinical studies? Opt. Lett. 27, 333–335 (2002).
Lim, Y. T. et al. Selection of quantum dot wavelengths for biomedical assays and imaging. Mol. Imaging 2, 50–64 (2003).
Carr, J. A. et al. Shortwave infrared fluorescence imaging with the clinically approved near-infrared dye indocyanine green. Proc. Natl Acad. Sci. USA 115, 4465–4470 (2018).
Bruns, O. T. et al. Next-generation in vivo optical imaging with short-wave infrared quantum dots. Nat. Biomed. Eng. 1, 0056 (2017).
Iwaisako, K. et al. Origin of myofibroblasts in the fibrotic liver in mice. Proc. Natl Acad. Sci. USA 111, E3297–E3305 (2014).
Delire, B., Stärkel, P. & Leclercq, I. Animal models for fibrotic liver diseases: what we have, what we need, and what is under development. J. Clin. Transl. Hepatol. 3, 53–66 (2015).
Scholten, D., Trebicka, J., Liedtke, C. & Weiskirchen, R. The carbon tetrachloride model in mice. Lab. Anim. 49, 4–11 (2015).
Geerts, A. M. et al. Comparison of three research models of portal hypertension in mice: macroscopic, histological and portal pressure evaluation. Int. J. Exp. Pathol. 89, 251–263 (2008).
Tag, C. et al. Induction of experimental obstructive cholestasis in mice. Lab. Anim. 49, 70–80 (2015).
Majno, G. & Joris, I. in Cells, Tissues, and Disease: Principles of General Pathology (eds Majno, G. & Joris, I.) 74–128 (Oxford University Press, 2004).
Terman, A. & Brunk, U. T. Lipofuscin. Int. J. Biochem. Cell Biol. 36, 1400–1404 (2004).
Rantakari, P. et al. Stabilin-1 expression defines a subset of macrophages that mediate tissue homeostasis and prevent fibrosis in chronic liver injury. Proc. Natl Acad. Sci. USA 113, 9298–9303 (2016).
Barden, H. The intragranular location of carboxyl groups in neuromelanin and lipofuscin in human brain and in meningeal melanosomes in mouse brain. J. Histochem. Cytochem. 34, 1271–1279 (1986).
Lillie, R. D. A Nile blue staining technic for the differentiation of melanin and lipofuscins. Stain Technol. 31, 151–153 (1956).
Evangelou, K. & Gorgoulis, V. G. in Oncogene-Induced Senescence: Methods and Protocols, Methods in Molecular Biology Vol. 1534 (ed. Nikiforov, M.) 111–119 (Humana Press, 2017).
Everson Pearse, A. G. in Histochemistry Theoretical and Applied Vol. 2, 898–928 (Churchill Livingstone, 1985).
Terman, A., Kurz, T., Navratil, M., Arriaga, E. A. & Brunk, U. T. Mitochondrial turnover and aging of long-lived postmitotic cells: the mitochondrial–lysosomal axis theory of aging. Antioxid. Redox Signal. 12, 503–535 (2010).
Seehafer, S. S. & Pearce, D. A. You say lipofuscin, we say ceroid: defining autofluorescent storage material. Neurobiol. Aging 27, 576–588 (2006).
Schnell, S. A., Staines, W. A. & Wessendorf, M. W. Reduction of lipofuscin-like autofluorescence in fluorescently labeled tissue. J. Histochem. Cytochem. 47, 719–730 (1999).
Erben, T., Ossig, R., Naim, H. Y. & Schnekenburger, J. What to do with high autofluorescence background in pancreatic tissues—an efficient Sudan black B quenching method for specific immunofluorescence labelling. Histopathology 69, 406–422 (2016).
Nazeer, S. S., Saraswathy, A., Shenoy, S. J. & Jayasree, R. S. Fluorescence spectroscopy as an efficient tool for staging the degree of liver fibrosis: an in vivo comparison with MRI. Sci. Rep. 8, 10967 (2018).
Kisseleva, T. et al. Myofibroblasts revert to an inactive phenotype during regression of liver fibrosis. Proc. Natl Acad. Sci. USA 109, 9448–9453 (2012).
Duffield, J. S. et al. Selective depletion of macrophages reveals distinct, opposing roles during liver injury and repair. J. Clin. Invest. 115, 56–65 (2005).
Liu, C. et al. Kupffer cells are associated with apoptosis, inflammation and fibrotic effects in hepatic fibrosis in rats. Lab. Invest. 90, 1805–1816 (2010).
Beljaars, L. et al. Hepatic localization of macrophage phenotypes during fibrogenesis and resolution of fibrosis in mice and humans. Front. Immunol. 5, 430 (2014).
Matsumoto, M. et al. An improved mouse model that rapidly develops fibrosis in non-alcoholic steatohepatitis. Int. J. Exp. Pathol. 94, 93–103 (2013).
Giannessi, F., Giambelluca, M. A., Scavuzzo, M. C. & Ruffoli, R. Ultrastructure of testicular macrophages in aging mice. J. Morphol. 263, 39–46 (2005).
Jara, M., Carballada, R. & Esponda, P. Age-induced apoptosis in the male genital tract of the mouse. Reproduction 127, 359–366 (2004).
Brunk, U. T. & Terman, A. Lipofuscin: mechanisms of age-related accumulation and influence on cell function. Free Radic. Biol. Med. 33, 611–619 (2002).
Softic, S. et al. Lipodystrophy due to adipose tissue-specific insulin receptor knockout results in progressive NAFLD. Diabetes 65, 2187–2200 (2016).
Friedman, S. L., Neuschwander-Tetri, B. A., Rinella, M. & Sanyal, A. J. Mechanisms of NAFLD development and therapeutic strategies. Nat. Med. 24, 908–922 (2018).
Alonso, C. et al. Metabolomic identification of subtypes of nonalcoholic steatohepatitis. Gastroenterology 152, 1449–1461 (2017).
Brunt, E. M., Janney, C. G., Bisceglie, A. M. Di, Neuschwander-Tetri, B. A. & Bacon, B. R. Nonalcoholic steatohepatitis—a proposal for grading and staging the histological lesions. Am. J. Gastroenterol. 94, 2467–2474 (1999).
Orchard, G.E., in Bancroft’s Theory and Practice of Histological Techniques (eds Suvarna, S. K., Layton, C. et al.) 239–270 (Elsevier, 2013).
Schmittgen, T. D. & Livak, K. J. Analyzing real-time PCR data by the comparative C
T method. Nat. Protoc. 3, 1101–1108 (2008).
Casteilla, L., Pénicaud, L., Cousin, B. & Calise, D. Choosing an adipose tissue depot for sampling: factors in selection and depot specificity. Methods Mol. Biol. 456, 23–38 (2008).
Schneider, C. A., Rasband, W. S. & Eliceiri, K. W. NIH Image to ImageJ: 25 years of image analysis. Nat. Methods 9, 671–675 (2012).