Home Journals MicroRNA-342-3p is a potent tumour suppressor in hepatocellular carcinoma

MicroRNA-342-3p is a potent tumour suppressor in hepatocellular carcinoma

Credits to the Source Link Daniel
MicroRNA-342-3p is a potent tumour suppressor in hepatocellular carcinoma

Background & Aims

Hepatocellular carcinoma (HCC) is a cancer with multiple etiologies, and widespread
prevalence. Largely refractory to current treatments, HCC is the fourth leading cause
of cancer related deaths, world-wide. MicroRNAs (miRNAs) are important regulators
in HCCs. We aimed to identify tumor suppressor miRNAs during tumor regression in a
conditional c-MYC driven mouse model (LT2/MYC) of HCC, and to evaluate their therapeutic
potential for HCC treatment


We performed miRNA expression profiling of developed and regressing LT2/MYC tumors,
and in-depth
in vitro gain- and loss-of-function analyses. Effect of adeno-associated virus vector (AAV)-mediated
miR-342-3p treatment was evaluated in three HCC mouse models


We identified miR-342-3p as a tumor suppressor miRNA in HCC, with increased expression
in regressing tumors. Forced miR-342-3p expression in hepatoma cells showed significantly
decreased cell proliferation, migration and colony formation.
In vivo administration of AAV-miR-342-3p led to significant attenuation of tumor development
and increased overall survival. We identified
Mct1 as a
bona fide target of miR-342-3p in HCC. We show the tumor suppressor role of miR-342-3p is executed
partly by modulating the lactate transport function of MCT1. Importantly, we find
miR-342-3p downregulated in tumors from HCC patients compared to matched non-tumor
tissues, inversely correlating with MCT1 expression. We observed similar findings
in TCGA-LIHC data.


In our study, we identified and validated miR-342-3p as a tumor suppressor miRNA,
in HCC. We demonstrated its therapeutic efficacy in significantly attenuating tumor
development, and prolonging survival, in different HCC mouse models. Identification
of miR-342-3p as an effective tumor suppressor opens a therapeutic avenue for miRNA-mediated
attenuation of HCC development.

Source Link

Related Articles

Leave a Comment

This website uses cookies to improve your experience. We will assume you are ok with this, but you can opt-out if you wish. Accept Read More

%d bloggers like this: