Home Journals Characterizing the Heterogeneity of Liver Cell Populations Under a NASH-Related Hepatotoxicant Using Single-Nuclei RNA Sequencing

Characterizing the Heterogeneity of Liver Cell Populations Under a NASH-Related Hepatotoxicant Using Single-Nuclei RNA Sequencing

Credits to the Source Link Pedro M. Rodrigues, Jesus M. Banales
Characterizing the Heterogeneity of Liver Cell Populations Under a NASH-Related Hepatotoxicant Using Single-Nuclei RNA Sequencing
Single-cell transcriptomic technologies constitute nowadays a powerful and robust tool to decode tissue heterogeneity and study gene expression at the individual cell level, constituting an essential approach to unravel cell-specific transcriptomic changes under pathological conditions as well as in response to exogenous agents, therefore paving the path to unravel the role of specific cell populations, mechanisms, and molecular events. Although single-cell RNA sequencing (scRNA-seq) provides robust and reliable data, some bias might be added due to cell isolation–induced transcriptional changes, while some cell types are more difficult to collect, being more vulnerable during the tissue dissociation process and therefore less represented in the final dataset. Furthermore, scRNA-seq requires freshly isolated cells, representing an important technical drawback. Recovery of RNA from isolated nuclei obtained from frozen tissue has already been described, presenting enough quality and sufficient amount to conduct transcriptomic profiling studies.

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  • Grindberg R.V.
  • Yee-Greenbaum J.L.
  • McConnell M.J.
  • Novotny M.
  • O’Shaughnessy A.L.
  • Lambert G.M.
  • Arauzo-Bravo M.J.
  • Lee J.
  • Fishman M.
  • Robbins G.E.
  • Lin X.
  • Venepally P.
  • Badger J.H.
  • Galbraith D.W.
  • Gage F.H.
  • Lasken R.S.
RNA-sequencing from single nuclei.